Protocol For Determining Radiopacity of Posts
I. Research Question: What is radiopacity of glass fiber posts?
II. Test Method
A. Prepare master tooth used to determine radiopacity of each post
1- Locate a freshly extracted human maxillary cuspid.
2- Cut crown off tooth at CEJ using Slow Speed Saw (Isomet).
3- Prepare root canal using Hedstrom endo files following traditional Step Back technique.
4- Place master gutta percha cone in tooth and laterally condense.
5- Drill 9 mm deep into tooth using Gates Glidden Drills # 2, 3, & 4.
6- Using 1.4 mm reamer, drill 9 mm deep in tooth.
7- Place a matrix band around tooth and inject core materials (Core Paste LC).
8- Light cure core material for 40 seconds using Optilux 500 (11mm tip, 725 mW/cm2)
9- Cut
hole in core material to allow insertion of post using high speed handpiece and
B. Take Radiograph
1- Seat test post in tooth.
2- Place test tooth (facial side up) along with aluminum step wedge (1-7mm) on Kodak E speed #2 film.
3- Place X-Ray head 1.5” above tooth. X-Ray setting are: 60 Kvp 7 ma .25 seconds.
4- Expose X-ray film.
5- Develop film.
C. Determine Gray Scale Measurement
1- Scan radiographs with consistent scanning settings using 500 dpi resolution.
2- Load image into software “Image Pro Plus”.
3- Calculate the gray scale value using line profile function. Use a line with at least 200 points of data and place center of post at the same location on every post.
4- Average the 200 points.
5- Calculate and average gray scale values of each of the 7 mm increments of the aluminum step wedge.
6- Using aluminum step wedge values assign mm of aluminum value to the post.
7- Divide the mm aluminum value by the diameter of the post at the location is was measured to get the % aluminum.